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prism 7000 sequencing detection system apparatus  (Thermo Fisher)


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    Structured Review

    Thermo Fisher prism 7000 sequencing detection system apparatus
    Prism 7000 Sequencing Detection System Apparatus, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/7000+sequence+detection+system+apparatus/pm37238670-99-38-44
    Average 90 stars, based on 1 article reviews
    prism 7000 sequencing detection system apparatus - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Sequencing:

    Article Title: Drastic changes in fecal and mucosa-associated microbiota in adult patients with short bowel syndrome.
    Article Snippet: Short bowel syndrome (SBS) is observed in Humans after a large resection of gut.. Since the remnant colon and its associated microbiota play a major role in the outcome of patients with SBS, we studied the overall qualitative and quantitative microbiota composition of SBS adult patients compared to controls.. The populationwas composed of 11 SBS type II patients (with a jejuno-colonic anastomosis) and 8 controls without intestinal pathology.

    Article Title: EFFECT OF MODE OF RIPENING ON ETHYLENE BIOSYNTHESIS DURING RIPENING OF DIPLOID BANANA (MUSA SPP.) FRUIT
    Article Snippet: Ripening is the main physiological process affecting banana (Musa spp.) fruit quality traits.. The progress of banana ripening process differs for fruit ripened on the plant versus green harvested bunches and depends on the treatment of the fruit after harvest.. We investigated the effect of the mode of ripening on ethylene biosynthesis of ‘IDN 110’ (Musa acuminata, AA genome) banana fruit ripened (a) in planta (On-Plant); (b) ex planta in air (Air-Fruit); or (c) after acetylene treatment (Ace-Fruit).

    Article Title: Expression of epidermal growth factor, fibroblast growth factor-2, and platelet-derived growth factor-A in the eutopic endometrium of women with endometriosis.
    Article Snippet: Aim: The objective of the present study was to compare the levels of expression of epidermal growth factor (EGF), fibroblast growth factor-2 (FGF-2), and platelet-derived growth factor-A (PDGF-A) mRNA in the eutopic endometrium of women with and without endometriosis.. Methods: Thirty-five women with advanced stage endometriosis and 31 control women were recruited.. Following isolation of total RNA from endometrial tissue and reverse transcription, cDNA samples were amplified to quantify the level of expression of EGF, FGF-2, PDGF-A.

    Article Title: Cathepsin B in Eutopic and Ectopic Endometrial Tissues of Patients with Endometriosis
    Article Snippet: .. Real-time PCR was carried out on approximately 50 ng cDNA utilizing an ABI 7000 Sequence Detection System apparatus (Applied Biosystems, Foster City, CA) in a 20-μl volume containing 1X SYBR Green PCR master mix (Applied Biosystems) and 10 pmole of the forward and reverse primers. .. All samples were assayed in triplicate, and each aliquot was transferred to wells of a MicroAmp optical 96-well reaction plate (Applied Biosystems).

    Article Title: The Firmicutes/Bacteroidetes ratio of the human microbiota changes with age
    Article Snippet: .. Real-time qPCR was performed using an ABI 7000 Sequence Detection System apparatus with system software version 1.2.3 (Applied-Biosystems) [ , ]. ..

    Article Title: Effects on in vitro fertilization-embryo transfer outcomes of vascular endothelial growth factor receptor-1, -2 and -3 in eutopic endometrial tissue of women with endometriosis.
    Article Snippet: Aim: This study was performed to assess the expression levels of vascular endothelial growth factor receptor (VEGFR)-1, -2 and -3 mRNA in eutopic endometrial tissue and their effects on in vitro fertilization–embryo transfer (IVF-ET) outcomes in women with endometriosis.. Materials and Methods: Twenty women with endometriosis III or IV (study group) and 20 with tubal infertility only (control group) were evaluated.. Controlled ovarian stimulation in all patients consisted of the long protocol using gonadotrophin-releasing hormone agonist.

    Article Title: Faecal D/L Lactate Ratio Is a Metabolic Signature of Microbiota Imbalance in Patients with Short Bowel Syndrome
    Article Snippet: Total bacterial DNA was extracted from 0.15 g to 0.2 g of faecal samples using a bead-beating method G'NOME DNA extraction kit (Mp Biomedicals, Illkirch) as described by Furet et al . Faecal DNAs were dissolved in 150 μl Tris-EDTA buffer and stored at −20°C. .. The real-time qPCR was performed using the ABI7000 sequence detection system apparatus and 7000 system software version 1.2.3 (Applied-Biosystems). .. Amplification and detection were carried out in 96-well plates with Taqman universal 2×PCR master mix or with Syber-Green PCR 2× master mix (Applied-Biosystems).

    Article Title: Alteration of PTGS2 Promoter Methylation in Chronic Periodontitis
    Article Snippet: RNA Isolation and Real-time PCR Total RNA was isolated from RNA later ® -treated gingival tissues with the use of an RNeasy Mini Kit (Qiagen). cDNA was then synthesized from 1 μg of total RNA with the Omniscript Kit (Qiagen) by random decamer primers (Applied Biosystems/Ambion). .. Real-time PCR was performed with 1 μL synthesized cDNA, TaqMan Universal PCR mix, and 20X on-demand primers (Applied Biosystems, Foster City, CA, USA) specific for the PTGS2 gene, in a 7000 Sequence Detection System apparatus (Applied Biosystems). ..

    Software:

    Article Title: Drastic changes in fecal and mucosa-associated microbiota in adult patients with short bowel syndrome.
    Article Snippet: Short bowel syndrome (SBS) is observed in Humans after a large resection of gut.. Since the remnant colon and its associated microbiota play a major role in the outcome of patients with SBS, we studied the overall qualitative and quantitative microbiota composition of SBS adult patients compared to controls.. The populationwas composed of 11 SBS type II patients (with a jejuno-colonic anastomosis) and 8 controls without intestinal pathology.

    Article Title: The Firmicutes/Bacteroidetes ratio of the human microbiota changes with age
    Article Snippet: .. Real-time qPCR was performed using an ABI 7000 Sequence Detection System apparatus with system software version 1.2.3 (Applied-Biosystems) [ , ]. ..

    Article Title: Faecal D/L Lactate Ratio Is a Metabolic Signature of Microbiota Imbalance in Patients with Short Bowel Syndrome
    Article Snippet: Total bacterial DNA was extracted from 0.15 g to 0.2 g of faecal samples using a bead-beating method G'NOME DNA extraction kit (Mp Biomedicals, Illkirch) as described by Furet et al . Faecal DNAs were dissolved in 150 μl Tris-EDTA buffer and stored at −20°C. .. The real-time qPCR was performed using the ABI7000 sequence detection system apparatus and 7000 system software version 1.2.3 (Applied-Biosystems). .. Amplification and detection were carried out in 96-well plates with Taqman universal 2×PCR master mix or with Syber-Green PCR 2× master mix (Applied-Biosystems).

    Polymerase Chain Reaction:

    Article Title: EFFECT OF MODE OF RIPENING ON ETHYLENE BIOSYNTHESIS DURING RIPENING OF DIPLOID BANANA (MUSA SPP.) FRUIT
    Article Snippet: Ripening is the main physiological process affecting banana (Musa spp.) fruit quality traits.. The progress of banana ripening process differs for fruit ripened on the plant versus green harvested bunches and depends on the treatment of the fruit after harvest.. We investigated the effect of the mode of ripening on ethylene biosynthesis of ‘IDN 110’ (Musa acuminata, AA genome) banana fruit ripened (a) in planta (On-Plant); (b) ex planta in air (Air-Fruit); or (c) after acetylene treatment (Ace-Fruit).

    Article Title: Expression of epidermal growth factor, fibroblast growth factor-2, and platelet-derived growth factor-A in the eutopic endometrium of women with endometriosis.
    Article Snippet: Aim: The objective of the present study was to compare the levels of expression of epidermal growth factor (EGF), fibroblast growth factor-2 (FGF-2), and platelet-derived growth factor-A (PDGF-A) mRNA in the eutopic endometrium of women with and without endometriosis.. Methods: Thirty-five women with advanced stage endometriosis and 31 control women were recruited.. Following isolation of total RNA from endometrial tissue and reverse transcription, cDNA samples were amplified to quantify the level of expression of EGF, FGF-2, PDGF-A.

    Article Title: Cathepsin B in Eutopic and Ectopic Endometrial Tissues of Patients with Endometriosis
    Article Snippet: .. Real-time PCR was carried out on approximately 50 ng cDNA utilizing an ABI 7000 Sequence Detection System apparatus (Applied Biosystems, Foster City, CA) in a 20-μl volume containing 1X SYBR Green PCR master mix (Applied Biosystems) and 10 pmole of the forward and reverse primers. .. All samples were assayed in triplicate, and each aliquot was transferred to wells of a MicroAmp optical 96-well reaction plate (Applied Biosystems).

    Article Title: Effects on in vitro fertilization-embryo transfer outcomes of vascular endothelial growth factor receptor-1, -2 and -3 in eutopic endometrial tissue of women with endometriosis.
    Article Snippet: Aim: This study was performed to assess the expression levels of vascular endothelial growth factor receptor (VEGFR)-1, -2 and -3 mRNA in eutopic endometrial tissue and their effects on in vitro fertilization–embryo transfer (IVF-ET) outcomes in women with endometriosis.. Materials and Methods: Twenty women with endometriosis III or IV (study group) and 20 with tubal infertility only (control group) were evaluated.. Controlled ovarian stimulation in all patients consisted of the long protocol using gonadotrophin-releasing hormone agonist.

    Article Title: Alteration of PTGS2 Promoter Methylation in Chronic Periodontitis
    Article Snippet: RNA Isolation and Real-time PCR Total RNA was isolated from RNA later ® -treated gingival tissues with the use of an RNeasy Mini Kit (Qiagen). cDNA was then synthesized from 1 μg of total RNA with the Omniscript Kit (Qiagen) by random decamer primers (Applied Biosystems/Ambion). .. Real-time PCR was performed with 1 μL synthesized cDNA, TaqMan Universal PCR mix, and 20X on-demand primers (Applied Biosystems, Foster City, CA, USA) specific for the PTGS2 gene, in a 7000 Sequence Detection System apparatus (Applied Biosystems). ..

    Reverse Transcription:

    Article Title: EFFECT OF MODE OF RIPENING ON ETHYLENE BIOSYNTHESIS DURING RIPENING OF DIPLOID BANANA (MUSA SPP.) FRUIT
    Article Snippet: Ripening is the main physiological process affecting banana (Musa spp.) fruit quality traits.. The progress of banana ripening process differs for fruit ripened on the plant versus green harvested bunches and depends on the treatment of the fruit after harvest.. We investigated the effect of the mode of ripening on ethylene biosynthesis of ‘IDN 110’ (Musa acuminata, AA genome) banana fruit ripened (a) in planta (On-Plant); (b) ex planta in air (Air-Fruit); or (c) after acetylene treatment (Ace-Fruit).

    Transferring:

    Article Title: EFFECT OF MODE OF RIPENING ON ETHYLENE BIOSYNTHESIS DURING RIPENING OF DIPLOID BANANA (MUSA SPP.) FRUIT
    Article Snippet: Ripening is the main physiological process affecting banana (Musa spp.) fruit quality traits.. The progress of banana ripening process differs for fruit ripened on the plant versus green harvested bunches and depends on the treatment of the fruit after harvest.. We investigated the effect of the mode of ripening on ethylene biosynthesis of ‘IDN 110’ (Musa acuminata, AA genome) banana fruit ripened (a) in planta (On-Plant); (b) ex planta in air (Air-Fruit); or (c) after acetylene treatment (Ace-Fruit).

    Real-time Polymerase Chain Reaction:

    Article Title: EFFECT OF MODE OF RIPENING ON ETHYLENE BIOSYNTHESIS DURING RIPENING OF DIPLOID BANANA (MUSA SPP.) FRUIT
    Article Snippet: Ripening is the main physiological process affecting banana (Musa spp.) fruit quality traits.. The progress of banana ripening process differs for fruit ripened on the plant versus green harvested bunches and depends on the treatment of the fruit after harvest.. We investigated the effect of the mode of ripening on ethylene biosynthesis of ‘IDN 110’ (Musa acuminata, AA genome) banana fruit ripened (a) in planta (On-Plant); (b) ex planta in air (Air-Fruit); or (c) after acetylene treatment (Ace-Fruit).

    Article Title: Expression of epidermal growth factor, fibroblast growth factor-2, and platelet-derived growth factor-A in the eutopic endometrium of women with endometriosis.
    Article Snippet: Aim: The objective of the present study was to compare the levels of expression of epidermal growth factor (EGF), fibroblast growth factor-2 (FGF-2), and platelet-derived growth factor-A (PDGF-A) mRNA in the eutopic endometrium of women with and without endometriosis.. Methods: Thirty-five women with advanced stage endometriosis and 31 control women were recruited.. Following isolation of total RNA from endometrial tissue and reverse transcription, cDNA samples were amplified to quantify the level of expression of EGF, FGF-2, PDGF-A.

    Article Title: Cathepsin B in Eutopic and Ectopic Endometrial Tissues of Patients with Endometriosis
    Article Snippet: .. Real-time PCR was carried out on approximately 50 ng cDNA utilizing an ABI 7000 Sequence Detection System apparatus (Applied Biosystems, Foster City, CA) in a 20-μl volume containing 1X SYBR Green PCR master mix (Applied Biosystems) and 10 pmole of the forward and reverse primers. .. All samples were assayed in triplicate, and each aliquot was transferred to wells of a MicroAmp optical 96-well reaction plate (Applied Biosystems).

    Article Title: The Firmicutes/Bacteroidetes ratio of the human microbiota changes with age
    Article Snippet: .. Real-time qPCR was performed using an ABI 7000 Sequence Detection System apparatus with system software version 1.2.3 (Applied-Biosystems) [ , ]. ..

    Article Title: Effects on in vitro fertilization-embryo transfer outcomes of vascular endothelial growth factor receptor-1, -2 and -3 in eutopic endometrial tissue of women with endometriosis.
    Article Snippet: Aim: This study was performed to assess the expression levels of vascular endothelial growth factor receptor (VEGFR)-1, -2 and -3 mRNA in eutopic endometrial tissue and their effects on in vitro fertilization–embryo transfer (IVF-ET) outcomes in women with endometriosis.. Materials and Methods: Twenty women with endometriosis III or IV (study group) and 20 with tubal infertility only (control group) were evaluated.. Controlled ovarian stimulation in all patients consisted of the long protocol using gonadotrophin-releasing hormone agonist.

    Article Title: Faecal D/L Lactate Ratio Is a Metabolic Signature of Microbiota Imbalance in Patients with Short Bowel Syndrome
    Article Snippet: Total bacterial DNA was extracted from 0.15 g to 0.2 g of faecal samples using a bead-beating method G'NOME DNA extraction kit (Mp Biomedicals, Illkirch) as described by Furet et al . Faecal DNAs were dissolved in 150 μl Tris-EDTA buffer and stored at −20°C. .. The real-time qPCR was performed using the ABI7000 sequence detection system apparatus and 7000 system software version 1.2.3 (Applied-Biosystems). .. Amplification and detection were carried out in 96-well plates with Taqman universal 2×PCR master mix or with Syber-Green PCR 2× master mix (Applied-Biosystems).

    Article Title: Alteration of PTGS2 Promoter Methylation in Chronic Periodontitis
    Article Snippet: RNA Isolation and Real-time PCR Total RNA was isolated from RNA later ® -treated gingival tissues with the use of an RNeasy Mini Kit (Qiagen). cDNA was then synthesized from 1 μg of total RNA with the Omniscript Kit (Qiagen) by random decamer primers (Applied Biosystems/Ambion). .. Real-time PCR was performed with 1 μL synthesized cDNA, TaqMan Universal PCR mix, and 20X on-demand primers (Applied Biosystems, Foster City, CA, USA) specific for the PTGS2 gene, in a 7000 Sequence Detection System apparatus (Applied Biosystems). ..

    SYBR Green Assay:

    Article Title: Expression of epidermal growth factor, fibroblast growth factor-2, and platelet-derived growth factor-A in the eutopic endometrium of women with endometriosis.
    Article Snippet: Aim: The objective of the present study was to compare the levels of expression of epidermal growth factor (EGF), fibroblast growth factor-2 (FGF-2), and platelet-derived growth factor-A (PDGF-A) mRNA in the eutopic endometrium of women with and without endometriosis.. Methods: Thirty-five women with advanced stage endometriosis and 31 control women were recruited.. Following isolation of total RNA from endometrial tissue and reverse transcription, cDNA samples were amplified to quantify the level of expression of EGF, FGF-2, PDGF-A.

    Article Title: Cathepsin B in Eutopic and Ectopic Endometrial Tissues of Patients with Endometriosis
    Article Snippet: .. Real-time PCR was carried out on approximately 50 ng cDNA utilizing an ABI 7000 Sequence Detection System apparatus (Applied Biosystems, Foster City, CA) in a 20-μl volume containing 1X SYBR Green PCR master mix (Applied Biosystems) and 10 pmole of the forward and reverse primers. .. All samples were assayed in triplicate, and each aliquot was transferred to wells of a MicroAmp optical 96-well reaction plate (Applied Biosystems).

    Article Title: Effects on in vitro fertilization-embryo transfer outcomes of vascular endothelial growth factor receptor-1, -2 and -3 in eutopic endometrial tissue of women with endometriosis.
    Article Snippet: Aim: This study was performed to assess the expression levels of vascular endothelial growth factor receptor (VEGFR)-1, -2 and -3 mRNA in eutopic endometrial tissue and their effects on in vitro fertilization–embryo transfer (IVF-ET) outcomes in women with endometriosis.. Materials and Methods: Twenty women with endometriosis III or IV (study group) and 20 with tubal infertility only (control group) were evaluated.. Controlled ovarian stimulation in all patients consisted of the long protocol using gonadotrophin-releasing hormone agonist.

    Synthesized:

    Article Title: Alteration of PTGS2 Promoter Methylation in Chronic Periodontitis
    Article Snippet: RNA Isolation and Real-time PCR Total RNA was isolated from RNA later ® -treated gingival tissues with the use of an RNeasy Mini Kit (Qiagen). cDNA was then synthesized from 1 μg of total RNA with the Omniscript Kit (Qiagen) by random decamer primers (Applied Biosystems/Ambion). .. Real-time PCR was performed with 1 μL synthesized cDNA, TaqMan Universal PCR mix, and 20X on-demand primers (Applied Biosystems, Foster City, CA, USA) specific for the PTGS2 gene, in a 7000 Sequence Detection System apparatus (Applied Biosystems). ..



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